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Milica Radisic, Ph.D.

AIMBE College of Fellows Class of 2015
In recognition of her innovative research on the design and laboratory development of heart tissue

Skin Cells ‘crawl’ Together To Heal Wounds Treated With Unique Hydrogel Layer

Via U. Toronto | December 14, 2016

Time may not heal all wounds, but a proprietary mix of peptides and gel developed by U of T Engineering researchers heals most.

A team led by Professor Milica Radisic (IBBME, ChemE) has demonstrated for the first time that their peptide-hydrogel biomaterial prompts skin cells to “crawl” toward one another, closing chronic, non-healing wounds often associated with diabetes, such as bed sores and foot ulcers.

The team tested their biomaterial on healthy cells from the surface of human skin, called keratinocytes, as well as on keratinocytes derived from elderly diabetic patients. They saw non-healing wounds close 200 per cent faster than with no treatment, and 60 per cent faster than treatment with a leading commercially used collagen-based product.

“We were happy when we saw the cells crawl together much faster with our biomaterial, but if it didn’t work with diabetic cells, that would have been the end of the story,” says Radisic. “But even the diabetic cells travelled much faster — that’s huge.”

Until now, most treatments for chronic wounds involved applying topical ointments that promote the growth of blood vessels to the area. But in diabetic patients, blood vessel growth is inhibited, making those treatments ineffective. Radisic and her team have been working with their special peptide — called QHREDGS, or Q-peptide for short — for almost 10 years. They knew it promoted survival of many different cell types, including stem cells, heart cells and fibroblasts (the cells that make connective tissues), but had never applied it to wound healing.

“We thought that if we were able to use our peptide to both promote survival and give these skin cells a substrate so they could crawl together, they would be able to close the wound more quickly,” says Radisic. “That was the underlying hypothesis.”

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U of T Engineering Professor Inducted into Canadian Academy of Engineering

Via U. Toronto | June 27, 2016

Milica Radisic is an international leader in the field of cardiovascular tissue engineering. She has pioneered the technique of electrical field stimulation for cultivation of functional heart tissue in the laboratory, leading to innovative new approaches to testing drugs in vitro on arrays of human tissues. Radisic’s groundbreaking research on the design and development of bioreactors for cardiac tissue engineering based on stem cell derived cardiomyocytes and biomaterials has led to accolades such as the NSERC E.W.R. Steacie Fellowship, the Engineers Canada Young Engineer Award, induction into the Royal Society of Canada’s College of New Scholars, Artists and Scientists, and inclusion as one of MIT Technology Review’s Top 35 Innovators under 35.

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CREATE Grants Boosts U of T Engineering Research

Via U. Toronto | April 14, 2016

Two collaborative research programs led by U of T Engineering professors have received major grants from the Natural Sciences and Engineering Research Council. The funding will help train a new generation of experts in leading edge technologies, from more efficient data transfers for cloud computing to new treatments for disease based on lab-grown human tissues.

Radisic and her team focus on growing human tissues outside the body. They recently unveiled the AngioChip, a system for growing realistic vascularized heart and liver tissues in the lab. These engineered tissues offer an effective way to test potential drug molecules and determine their effect on these organs, an application that is already being commercialized through the spin-off company TARA Biosystems. Eventually, they could be implanted into the body to repair or replace damaged organs.

The new funding will leverage the success of AngioChip’s commercialization. “In contrast to other CREATE programs that provide training through industrial internships, our training here will focus on developing a company,” said Radisic. “Our students will learn how to apply for patents, develop a business plan, make an effective fundraising pitch and more.”

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Human-Powered Vehicles and Tissue Engineering

Via U. Toronto | April 1, 2016

Professor Milica Radisic (IBBME, ChemE) shared several discoveries from her lab, including a new scaffold that supports the growth of realistic heart and liver tissues outside the body. Such tissues provide an ideal platform for testing new drugs for side effects, but the hope is that one day they will allow for the replacement of damaged human organs. “Organ regeneration is certainly something we will achieve in the future,” said Radisic.

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‘Person-on-a-chip’ — U of T engineers Create Lab-Grown Heart and Liver Tissue for Drug Testing and More

Via U. Toronto | March 7, 2016

Researchers at U of T Engineering have developed a new way of growing realistic human tissues outside the body. Their “person-on-a-chip” technology, called AngioChip, is a powerful platform for discovering and testing new drugs, and could eventually be used to repair or replace damaged organs.

Professor Milica Radisic (IBBME, ChemE), graduate student Boyang Zhang and their collaborators are among those research groups around the world racing to find ways to grow human tissues in the lab, under conditions that mimic a real person’s body. They have developed unique methods for manufacturing small, intricate scaffolds for individual cells to grow on. These artificial environments produce cells and tissues that resemble the real thing more closely than those grown lying flat in a petri dish.

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Lab-Grown Heart Cells To Improve Drug Safety

Via U. Toronto | February 22, 2016

Under a microscope at U of T Engineering, heart cells are pulsing together in a petri dish. For Professor Milica Radisic (IBBME, ChemE) and her team, it’s just another day in the lab.

Although they are grown outside the body, the human tissues engineered in Radisic’s lab look and behave just like the real thing. Now, thanks to a new startup company co-founded by Radisic, they are helping drug companies test their products for dangerous side effects.

Testing on lab-grown tissues is one way for pharmaceutical companies to get detailed information on how a drug will perform in the body before proceeding to clinical trials. It avoids many of the ethical issues associated with animal testing and is also much less costly. The hope is that by flagging negative side effects early in the testing process, the new method will help companies avoid the prospect of a drug withdrawals.

One well-known example is the case of Vioxx (also known as rofecoxib), a drug widely used to treat chronic pain. In 2004, Vioxx was withdrawn from the market over concerns about its connection to increased risk of heart attack and stroke. “The company set up a $5-billion fund to compensate people, but there were about 27,000 deaths,” says Radisic. “You can’t measure that with money.”

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New “Tissue Velcro” Could Help Repair Damaged Hearts

Via U. Toronto | August 28, 2015

Engineers at the University of Toronto just made assembling functional heart tissue as easy as fastening your shoes. The team has created a biocompatible scaffold that allows sheets of beating heart cells to snap together just like Velcro™.

“One of the main advantages is the ease of use,” says Professor Milica Radisic (ChemE, IBBME), who led the project. “We can build larger tissue structures immediately before they are needed, and disassemble them just as easily. I don’t know of any other technique that gives this ability.”

Growing heart muscle cells in the lab is nothing new. The problem is that too often, these cells don’t resemble those found in the body. Real heart cells grow in an environment replete with protein scaffolds and support cells that help shape them into long, lean beating machines. In contrast, lab-grown cells often lack these supports, and tend to be amorphous and weak. Radisic and her team focus on engineering artificial environments that more closely imitate what cells see in the body, resulting in tougher, more robust cells.

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Milica Radisic, Ph.D. To be Inducted into Medical and Biological Engineering Elite

Via AIMBE | March 5, 2015

WASHINGTON, D.C.— The American Institute for Medical and Biological Engineering (AIMBE) has announced the pending induction of Milica Radisic, Ph.D., Professor, Institute of Biomaterials and Biomedical Engineering, University of Toronto, to its College of Fellows. Dr. Radisic was nominated, reviewed, and elected by peers and members of the College of Fellows In recognition of her innovative research on the design and laboratory development of heart tissue.

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With Wires and Electrodes, Tara Bio Builds a Heart on a Chip

Via Xconomy | November 12, 2014

It’s been pretty clear for some time that there’s a big need to improve the efficiency of drug R&D. By industry’s count, drugs cost over $1 billion to develop, and most of them fail. A big reason why is the preclinical studies in petri dishes and animals don’t accurately predict how a drug will behave in humans.

That problem has prompted a slew of companies to innovate new ways to test drugs in a more human-representative fashion, and perhaps even change how preclinical testing is done. The latest to come along is Tara Biosystems, a New York-based Columbia University spinout whose founders say they’ve found a novel, stem-cell based approach that can simulate how a human heart would react to a drug.

Tara recently started up with the help of seed funding from New York-based Harris & Harris Group (NASDAQ: TINY), a publicly-traded venture firm that invests in early-stage companies. Misti Ushio, a managing director and executive VP of Harris & Harris, is leading the company, which is being incubated within the firm.

Regulatory filings show that Harris & Harris has invested around $300,000 in Tara. Ushio says the seed funding gives the company about a year’s worth of runway to prove itself—to validate its technology, convince pharmaceutical companies of its worth, and essentially earn an additional investment, like a full-fledged Series A round.

Tara is based on the work of two researchers: Columbia professor Gordana Vunjak-Novakovic and University of Toronto professor Milica Radisic. They met several years ago at Bob Langer’s lab at MIT and have since worked together to turn stem cells into mature heart tissue that can be tested as if it were an adult heart.

At the core of Tara’s technology are what the founders call “biowire.” Two parallel polymer wires are attached to the wells of a 96-well microplate (pictured above), a common piece of research equipment; the wells function as small test tubes.

The idea is that a researcher would put stem cells into the wells, and then mature them with the help of electrical stimulation. The stem cells grab on to the polymer wires and grow across them as they mature, eventually forming what amounts to tiny micro-hearts, with all of the different cell types that constitute adult heart tissue. Those tiny hearts beat, and pull on the wires when they do. The wires allow for tension, which is important, because by measuring how those wires move, a researcher could determine how the heart contracts, and what, specifically, is causing it to contract in the way it does.

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Six Ways U Of T Researchers Are Engineering Your Health

Via U. of Toronto | October 30, 2014

Like a scene lifted from Frankenstein, Professor Milica Radisic’s (IBBME, ChemE) “biowire” platform treats immature human cardiac cells, derived from stem cells, to cycles of electric pulses—a process that encourages growth at the same rate as a developing fetus. The result: mature heart tissue that meets a major need in the pharma industry, which, until now, has been unable to test medications on live human heart tissue. Grown along sutures, the heart tissue may also eventually be developed as cardiac patches that, derived from a patient’s own cells, won’t cause rejection issues. Radisic is currently developing the commercial possibilities of this remarkable technology.

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