Caltech researchers have developed a technique that combines fluorescence and ultrasound to peer through opaque media, such as biological tissue.
“We hope that one day this method can be deployed to extend the operating depth of fluorescence microscopy and help image fluorescent labeled cells deep inside living animals,” says Changhuei Yang, Thomas G. Myers Professor of Electrical Engineering, Bioengineering, and Medical Engineering, and senior author of a paper about the technique, dubbed fluorescence and ultrasound-modulated light correlation (FLUX for short, with the “X” for “correlation”), that was published on May 11 in Nature Photonics.
Scientists have long used glowing fluorescent probes for biological imaging; for example, tagging specific neurons that are then imaged for neuroscience studies. While fluorescence microscopy can provide nano- to microscale resolution, the resolution decreases rapidly along depth into biological tissue, because most biological tissue is opaque. As such, imaging fluorescent targets with high resolution, deep in tissue, has been a challenging task similar to seeing through fog… Continue reading.